Sdc-1 Knockdown in MDA-MB-231 Cells

MDA-MB-231 cells were cultured in six-well plates in growth media at a density of 350,000 cells/well. Then, 24 h after seeding, they were transfected with 20 nM pre-validated siRNA (Ambion life technologies, Cambridgeshire, UK) targeting exon 2 of Sdc-1 (NM_002997.4) to achieve the knockdown of Sdc-1 expression or a Silencer^TM Select negative control siRNA (Ambion) using Dharmafect reagent (DharmaconTM, Lafayette, CO, USA), according to the supplier’s protocols and as described previously, using a ratio of 2 µL/mL Dharmacon reagent and 20 pmol/mL siRNA [3 (link),4 (link)].

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Hassan N., Bückreiß N., Efing J., Schulz-Fincke M., König P., Greve B., Bendas G, & Götte M. (2023). The Heparan Sulfate Proteoglycan Syndecan-1 Triggers Breast Cancer Cell-Induced Coagulability by Induced Expression of Tissue Factor. Cells, 12(6), 910.

Publication 2023

pre-validated siRNA

Cells Exon Growth media Mda mb 231 cells Sirna

Corresponding Organization : University Hospital Münster

Other organizations : Cairo University, University of Bonn

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Variable analysis

independent variables

Transfection with 20 nM pre-validated siRNA targeting exon 2 of Sdc-1 (NM_002997.4) to achieve the knockdown of Sdc-1 expression
Transfection with Silencer™ Select negative control siRNA

dependent variables

Sdc-1 expression

control variables

MDA-MB-231 cells cultured in growth media at a density of 350,000 cells/well
Transfections performed using Dharmafect reagent according to supplier's protocols and as described previously, using a ratio of 2 µL/mL Dharmacon reagent and 20 pmol/mL siRNA

controls

Silencer™ Select negative control siRNA as a negative control

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