For immunoblot analysis, whole-cell lysates were prepared 3 h and 24 h after addition of the drugs according to standard procedures. Samples equivalent to 20-40 μg of protein were separated using 4-12% SDS-polyacrylamide pre-cast gels (Invitrogen, Karlsruhe, Germany) and transferred to nitrocellulose membranes, as described previously [46 (link)].
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