Immunoblot Analysis of Cell Lysates
Corresponding Organization :
Other organizations : Universitätsklinikum Würzburg, University of Würzburg, Fraunhofer Institute for Biomedical Engineering, Saarland University
Variable analysis
- Addition of the drugs
- Changes in protein expression levels
- Protein amount (20-40 μg) used for SDS-PAGE and immunoblotting
- SDS-polyacrylamide gel used for protein separation (4-12% pre-cast gels)
- Transfer of proteins to nitrocellulose membranes
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
Annotations
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