Bacterial DNA and RNA Extraction

Genomic DNA from 1 ml of bacterial cultures was extracted with the GeneElute Bacterial Genomic DNA Kit (SIGMA), quantified using NanoDrop spectrophotometer (Nanodrop Technologies), and its integrity was evaluated by agarose gel electrophoresis. For RNA-seq experiments, total RNA was extracted and treated as previously described [40] (link). Total RNA extraction for qRT-PCR was performed using RNeasy minikit (QIAGEN) from 1 ml of BtCDC272 overnight cultures (ca. 16 hours growth, Figure S1). RNA samples were checked by agarose gel electrophoresis to assess the lack of degradation and then quantified with NanoDrop spectrophotometer. Genomic DNA degradation and reverse transcription were performed on 1 μg of total RNA using QuantiTect Reverse Transcription kit (QIAGEN).