To identify the subcellular location of MeWRKY IIas, the full-length cDNA sequences of six MeWRKY IIas were cloned and linked into pGBT vector and then transferred into cassava protoplast (Wu et al., 2017 (link)). The cell nucleus was stained with Hoechst 33,342 solution (Solarbio, Beijing, China). The green fluorescent signals were observed with confocal laser-scanning microscope (TCS SP8, Leica, Heidelberg, Germany).
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