Bone Biomarker Measurement Methods

Plasma concentration of glucose was measured using the glucose oxidase method (Analox GM7 or GM9; Analox Instruments Ltd., London, UK) and serum insulin was determined by time-resolved immunofluorometric assay (AutoDELFIA; PerkinElmer Life and Analytical Sciences , Waltham, MA). Bone resorption was assessed by C-terminal crosslinked telopeptides of type I collagen (CTX, IDS-iSYS CTX-I ELISA, CrossLaps®) and bone formation by intact N-terminal propeptide of type I collagen (PINP, IDS-iSYS Intact PINP assay), both from IDS Ltd., East Boldon, UK. Serum total osteocalcin was determined with 2-site immunoassay based on monoclonal antibodies 2H9 and 6F9 using previously described protocol (23 (link)).

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Pham T.T., Ivaska K.K., Hannukainen J.C., Virtanen K.A., Lidell M.E., Enerbäck S., Mäkelä K., Parkkola R., Piirola S., Oikonen V., Nuutila P, & Kiviranta R. (2020). Human Bone Marrow Adipose Tissue is a Metabolically Active and Insulin-Sensitive Distinct Fat Depot. The Journal of Clinical Endocrinology and Metabolism, 105(7), 2300-2310.

Publication 2020

Analox GM7 AutoDELFIA

Assay Bone formation Bone resorption C terminal telopeptides of type i collagen Crosslaps Elisa Glucose Glucose oxidase Immunoassay Insulin Monoclonal antibodies Osteocalcin Pinp Plasma Serum Time resolved immunofluorometric assay Type i collagen

Corresponding Organization :

Other organizations : University of Turku, Turku PET Centre, University of Gothenburg, Turku University Hospital

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Variable analysis

independent variables

Plasma concentration of glucose measured using the glucose oxidase method (Analox GM7 or GM9; Analox Instruments Ltd., London, UK)
Serum insulin determined by time-resolved immunofluorometric assay (AutoDELFIA; PerkinElmer Life and Analytical Sciences, Waltham, MA)

dependent variables

Bone resorption assessed by C-terminal crosslinked telopeptides of type I collagen (CTX, IDS-iSYS CTX-I ELISA, CrossLaps®)
Bone formation by intact N-terminal propeptide of type I collagen (PINP, IDS-iSYS Intact PINP assay)
Serum total osteocalcin determined with 2-site immunoassay based on monoclonal antibodies 2H9 and 6F9

control variables

Not explicitly mentioned

positive controls

Not specified

negative controls

Not specified

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