Biomarker Analysis in Clinical Samples

Blood and urine samples were analysed at a single clinical laboratory at the Royal Derby Hospital. Creatinine was measured using the Jaffe reaction and was standardised to IDMS methods. eGFR was calculated using the MDRD equation [11 (link)] at the time of recruitment, but for analysis, this was changed to the more accurate chronic kidney disease epidemiology (CKD-EPI) equation [12 (link)], published after recruitment commenced. Additionally, serum was analysed for standard electrolytes and bone mineral profile. Urinary albumin was measured using an immunoturbidimetric assay (‘Tina-quant’, Roche Diagnostics, Mannheim, Germany) on a Roche Modular system. Urine albumin to creatinine ratio (UACR) was measured on three urine samples from each participant, and a mean value was used for analysis. Serum high-sensitivity C reactive protein (CRP) (hsCRP^™, Roche Diagnostics, Newhaven, UK) was measured using a Roche Modular P Analyser (Roche Diagnostics) at The Binding Site Group laboratories, Birmingham, UK.

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Shardlow A., McIntyre N.J., Kolhe N.V., Nellums L.B., Fluck R.J., McIntyre C.W, & Taal M.W. (2020). The association of skin autofluorescence with cardiovascular events and all-cause mortality in persons with chronic kidney disease stage 3: A prospective cohort study. PLoS Medicine, 17(7), e1003163.

Publication 2020

Tina-quant hsCRP™, Roche Modular P Analyser

Albumin Binding site Blood Bone Chronic kidney disease Clinical laboratory Creatinine Diagnostics Egfr Electrolytes Hscrp Immunoturbidimetric assay Mineral Serum Tina Urine

Corresponding Organization : Royal Derby Hospital

Other organizations : London Health Sciences Centre, Victoria Hospital, Western University

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Variable analysis

independent variables

Not explicitly mentioned

dependent variables

Creatinine
EGFR (estimated glomerular filtration rate)
Serum electrolytes
Bone mineral profile
Urinary albumin
Urine albumin to creatinine ratio (UACR)
Serum high-sensitivity C reactive protein (hsCRP)

control variables

Blood and urine samples were analysed at a single clinical laboratory at the Royal Derby Hospital
Creatinine was measured using the Jaffe reaction and was standardised to IDMS methods
EGFR was calculated using the MDRD equation at the time of recruitment, but for analysis, this was changed to the more accurate chronic kidney disease epidemiology (CKD-EPI) equation
Urinary albumin was measured using an immunoturbidimetric assay ('Tina-quant', Roche Diagnostics, Mannheim, Germany) on a Roche Modular system
Urine albumin to creatinine ratio (UACR) was measured on three urine samples from each participant, and a mean value was used for analysis
Serum high-sensitivity C reactive protein (CRP) (hsCRP™, Roche Diagnostics, Newhaven, UK) was measured using a Roche Modular P Analyser (Roche Diagnostics) at The Binding Site Group laboratories, Birmingham, UK

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