Invasion Assay for Spheroid Cells

The invasion test was performed as described by Martínez-Rodríguez et al. [84 (link)] with some modifications. Briefly, 1.2 × 10³ cells were seeded per well, and after the formation of the spheroid, it was treated with 800 and 1000 μg/mL of CSE and 9.94 μg/mL of cisplatin for 24 h. Subsequently, 100 μL of Matrigel^® Matrix (Corning^®) per well and then RPMI medium was added. The invasion was determined using an inverted Zeiss Axiovert 25 microscope (Carl Zeiss) and ImageJ software and calculated by measuring the area and perimeter between the spheroids’ edge and the invading cells’ edge.

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Agena R., Cortés-Sánchez A.D., Hernández-Sánchez H., Álvarez-Salas L.M., Martínez-Rodríguez O.P., García V.H, & Jaramillo Flores M.E. (2023). Pro-Apoptotic Activity and Cell Cycle Arrest of Caulerpa sertularioides against SKLU-1 Cancer Cell in 2D and 3D Cultures. Molecules, 28(11), 4361.

Publication 2023

Matrigel® Matrix Zeiss Axiovert 25 microscope

Cells Cisplatin Matrigel Microscope Perimeter

Corresponding Organization :

Other organizations : Instituto Politécnico Nacional, Consejo Nacional de Humanidades, Ciencias y Tecnologías, Center for Research and Advanced Studies of the National Polytechnic Institute

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Variable analysis

independent variables

Concentration of cigarette smoke extract (CSE) (800 and 1000 μg/mL)
Concentration of cisplatin (9.94 μg/mL)

dependent variables

Invasion of cells, measured by the area and perimeter between the spheroids' edge and the invading cells' edge

control variables

Cell seeding density (1.2 × 10^3 cells per well)
Incubation time (24 h)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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