NIH/3T3 or primary cells were starved in DMEM without FBS for 24 h, and then cultured in DMEM with 5% FBS and 5 nM hTGF-β1 (Peprotech, 500-M66) or 10 nM BI6727 (Selleck, S2235) for three days. Immunofluorescence was performed as previously described [44 (link)].
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