Example 4

Detection of MRSA was tested in a model of co-infection with excess methicillin-resistant Staphylococcus epidermidis (MRSE). In the specific model tested, MRSA (GP1822) was present at 10′ CFU/mL, either alone (MRSA Control); with MRSE at 106 CFU/mL (MRSA_MRSE 1e6); or with MRSE at 107 CFU/mL (MRSA_MRSE 1e7) (FIG. 4). Results showed no loss in detection under any condition, with positivity seen in 12/12 samples tested. Thus, the assay was able to detect MRSA in the presence of 1,000-fold and 10,000-fold excesses of MRSE.

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