To evaluate the changes of collagen deposition in lungs, Masson’s trichrome staining was performed as previously described [22 (link)]. Immunohistochemical staining was performed as previously described with minor modifications on the paraffin-embedded lung tissues [21 , 22 (link)]. N-Histofine MOUSESTAIN KIT (Nichirei Biosciences Inc., # 414321) was used for immunohistochemical staining of mouse lung sections.
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