All antibodies used for IHC and IF are listed in Figure Captions. Fixed,
frozen brain tissue was sectioned to 14 μm thickness, and prepared for
immunofluorescence using methods described elsewhere.[69 (link)] Sections were imaged on a Zeiss
Axiovision inverted microscope. For IHC, tissues were processed at the Emory
Winship Pathology Core Lab, as described elsewhere.[41 (link)] Tissues from paraffin-embedded blocks
were sectioned at 5 μm thickness. IHC was performed using DAB chromogenic
kit (Wako) following the manufacturer’s protocol. Whole-slide scanning
was done using a Hamamatsu Nanozoomer 2.0 HT
frozen brain tissue was sectioned to 14 μm thickness, and prepared for
immunofluorescence using methods described elsewhere.[69 (link)] Sections were imaged on a Zeiss
Axiovision inverted microscope. For IHC, tissues were processed at the Emory
Winship Pathology Core Lab, as described elsewhere.[41 (link)] Tissues from paraffin-embedded blocks
were sectioned at 5 μm thickness. IHC was performed using DAB chromogenic
kit (Wako) following the manufacturer’s protocol. Whole-slide scanning
was done using a Hamamatsu Nanozoomer 2.0 HT
