Bacterial Identification and Antimicrobial Susceptibility

According to the manufacturer’s instructions, bacterial identification and antimicrobial susceptibility tests were performed with Vitek 2 (bioMerieux, Marcy l’Etoile, France). PA suspensions from pure cultures were inoculated in 3 mL of a 0.45% NaCl solution and adjusted to a McFarland standard of 0.5, using a Densicheck (bioMérieux, Marcy l’Etoile, France). The results of antimicrobial susceptibility tests were interpreted according to EUCAST guidelines (https://www.eucast.org/clinical_breakpoints, accessed on 13 February 2022). The antibiotics tested included piperacillin/tazobactam (2/4–48/8 μg/mL), ceftazidime (0.25–32 μg/mL), cefepime (0.25–32 μg/mL), imipenem (1–12 μg/mL), amikacin (2–48 μg/mL), gentamicin (4–32 μg/mL), ciprofloxacin (0.06–1 μg/mL), and levofloxacin (0.12–8 μg/mL) [27 (link)].