QRT-PCR was performed as previously described(13 (link)). TaqMan gene expression assay primer/probes were purchased from Applied Biosystems (Foster City, CA) for cyclophilin A (Hs99999904_m1), OGT (Hs00914634_g1), SIRT1 (Hs01009005_m1), MMP-9 (Hs00234579_m1).
Protocol detail
Quantitative Real-Time PCR Analysis
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Corresponding Organization :
Other organizations : Drexel University, Harvard University
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Variable analysis
independent variables
- QRT-PCR
- Expression of cyclophilin A
- Expression of OGT
- Expression of SIRT1
- Expression of MMP-9
- Primer/probes purchased from Applied Biosystems
- Positive controls not explicitly mentioned
- Negative controls not explicitly mentioned
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