Western Blot Analysis of FADS1 and FADS2

Western blots were performed as previously described [39 (link)]. The primary antibodies were FADS1 (1:1000; ab126706), FADS2 (1:1000; Proteintech 28034-1-AP), GAPDH (1:5000; Affinity AF7021), actin (1:5000; Proteintech 60008-1-Ig). Then, the corresponding secondary antibodies were incubated at room temperature for 1 h. The bound antibody was expressed using the ECL method and detected with the BIO-RID imaging system (BIO-RAD, Hercules, CA, USA). The grayscale values of all bands were analyzed with Image Lab (Image J 1.53C, Millipore, Burlington, MA, USA) software.