Lamina propria cells were isolated and analyzed by flow cytometry as previously described 44 (link) and in
Isolation and Analysis of Immune Cells
Lamina propria cells were isolated and analyzed by flow cytometry as previously described 44 (link) and in
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Corresponding Organization : Cincinnati Children's Hospital Medical Center
Other organizations : University of Virginia
Variable analysis
- Intra-cardiac puncture for peripheral blood collection
- Flushing single femur and tibia with ice cold PBS containing 2% fetal bovine serum (FBS) for bone marrow cell harvesting
- Total and differential cell count determined using a Hemavet hematology analyzer
- Apoptosis of cecal tissue neutrophils enumerated using Annexin V/7AAD-apoptosis assay kit
- EDTA microtainers (BD Biosciences, CA, USA) for peripheral blood collection
- Fetal bovine serum (FBS, Thermo Fisher Scientific, MA, USA) used in PBS for bone marrow cell harvesting
- Not explicitly mentioned
- Not explicitly mentioned
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