Swine Testis (ST) and HIEC-6 cells were maintained in Dulbecco’s modified Eagle medium (DMEM) (Gibco, Billings, MT, USA) supplemented with 10% fetal bovine serum (FBS) (Gibco, USA) and a 1% antibiotic antimycotic solution (Solarbio, Beijing, China) at 37 °C in a humidified atmosphere of 5% CO2 [21 (link)]. ST and HIEC-6 cells were purchased from ATCC. The anti-PDCoV N rabbit monoclonal antibody was prepared by our laboratory. HRP-conjugated goat anti-rabbit IgG (A0208, Beyotime, Shanghai, China) was procured from Beyotime. GoTaq® QpcR Master Mix (A600A, Promega, Madison, WI, USA) was acquired from Promega.
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