Mice were inoculated with ID8 cells and on day 25 received either 200 μg hamster IgG (Jackson ImmunoResearch Laboratories, West Grove, PA) or 200 μg G4 clone PD-1–blocking monoclonal antibody i.p. as described (21 (link), 22 (link)). Mice were treated until moribund up to 11 treatments (8 - 11 times up to 7 weeks). IL-10 neutralizing antibody (BioLegend, San Diego, CA) and IL-10R antagonist antibody (BD Pharmingen, San Diego, CA) were injected i.p alternating between the two antibodies at a concentration of 100 μg and 200 μg per mouse, respectively. The same amount of isotype matched antibodies (Rat IgG1κ or Rat IgG1χ) from the respective suppliers was injected in the control groups. Tumor and ascites were harvested as necessary when mice were moribund.
Protocol detail
Investigating PD-1 Blockade and IL-10 Inhibition in Murine Ovarian Cancer
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Antibodies
Antibodies isotype
Antibody
Ascites
Cells
Clone
Hamster
Il 10
Il 10r
Mice
Monoclonal antibody
Neutralizing antibody
Tumor
Corresponding Organization : Vaccine & Gene Therapy Institute of Florida
Other organizations : University of Arkansas for Medical Sciences
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Variable analysis
independent variables
- Treatment with 200 μg hamster IgG (Jackson ImmunoResearch Laboratories, West Grove, PA)
- Treatment with 200 μg G4 clone PD-1–blocking monoclonal antibody i.p.
- Tumor growth
- Ascites formation
- Mice inoculated with ID8 cells
- Mice treated until moribund up to 11 treatments (8 - 11 times up to 7 weeks)
- IL-10 neutralizing antibody (BioLegend, San Diego, CA) and IL-10R antagonist antibody (BD Pharmingen, San Diego, CA) injected i.p alternating between the two antibodies at a concentration of 100 μg and 200 μg per mouse, respectively
- Isotype matched antibodies (Rat IgG1κ or Rat IgG1χ) from the respective suppliers injected in the control groups
- Positive control: Treatment with 200 μg hamster IgG (Jackson ImmunoResearch Laboratories, West Grove, PA)
- Negative control: Treatment with isotype matched antibodies (Rat IgG1κ or Rat IgG1χ) from the respective suppliers
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