For metabolome analysis, cells were lysed and metabolites were extracted from sediments in the FTRs in a methanol:chloroform mixture (1:2, v:v; -20C°) for 30 min under sonication. The metabolites in the supernatants were analysed by GC-QTOF-MS and LC-QTOF-MS (both Agilent Technologies) following Godzien, et al. 51 (link) and Kind, et al. 52 (link) with slight modifications. Volatile fatty acid concentration in FTR effluent was determined using SPME GC-MS. A HP-5MS non polar column (Agilent Technologies, Mount Waverley, Australia) of dimensions 30 m x 0.25 mm ID x 0.25 μm film thickness was used for separation in conjunction with splitless injection mode and H2 carrier gas. The method was optimized by exposing Carboxen/Polydimethylsiloxane SPME fibre to 10 mL of sample seawater headspace at 50°C for 60 min with 2g NaCl and acid addition prior to injection.
Protocol detail
Metabolomic Analysis of FTR Samples
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Acid
Cells
Chloroform
Fibre
Gc ms
Metabolome
Methanol
Nacl
Polydimethylsiloxane
Spme
Volatile fatty acid
Corresponding Organization :
Other organizations : Monash University, University of Southern Denmark, Texas A&M University at Galveston
Top 5 similar protocols
Variable analysis
independent variables
- Lysis method: Cells were lysed and metabolites were extracted from sediments in the FTRs in a methanol:chloroform mixture (1:2, v:v; -20C°) for 30 min under sonication.
- GC-QTOF-MS and LC-QTOF-MS analysis: Metabolites in the supernatants were analysed by GC-QTOF-MS and LC-QTOF-MS.
- SPME GC-MS analysis: Volatile fatty acid concentration in FTR effluent was determined using SPME GC-MS.
- Metabolite profile: Metabolites extracted from the cells were analyzed by GC-QTOF-MS and LC-QTOF-MS.
- Volatile fatty acid concentration: Volatile fatty acid concentration in FTR effluent was determined using SPME GC-MS.
- GC-MS column: A HP-5MS non polar column (Agilent Technologies, Mount Waverley, Australia) of dimensions 30 m x 0.25 mm ID x 0.25 μm film thickness was used for separation in conjunction with splitless injection mode and H2 carrier gas.
- SPME method: The method was optimized by exposing Carboxen/Polydimethylsiloxane SPME fibre to 10 mL of sample seawater headspace at 50°C for 60 min with 2g NaCl and acid addition prior to injection.
- No positive or negative controls were explicitly mentioned in the provided information.
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!