Cultivation of Anaerobic Gut Bacteria

The bacterial strains used in this study were Bacteroides fragilis (32-6-I 11 MRS AN), Escherichia coli K12 (ATCC 25404), Bifidobacterium longum subsp. infantis (ATCC 15697), Bacteroides caccae (ATCC 43185), Prevotella intermedia (ATCC 25611), Clostridium beijerinckii (ATCC 51743), Ruminococcus bromii (ATCC 27255), Akkermansia muciniphila (ATCC BAA-835), and Enterococcus faecalis (SF24397). Each bacterium was originally isolated from the human gut, with the exception of P. intermedia, which comes from the human oral cavity. All bacteria were cultured anaerobically in an anerobic chamber (Coy Laboratory Products; 5% H2, 20% CO2, 75% N2) at 37°C overnight directly from frozen (−80°C) glycerol stocks, in autoclaved reduced Bovine Heart Infusion broth (rBHI; Oxoid).

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Beauchemin E.T., Hunter C, & Maurice C.F. (2023). Actively replicating gut bacteria identified by 5-ethynyl-2’-deoxyuridine (EdU) click chemistry and cell sorting. Gut Microbes, 15(1), 2180317.

Publication 2023

anerobic chamber

Akkermansia muciniphila Anerobic Bacteria Bacteroides caccae Bacteroides fragilis Bifidobacterium longum subsp infantis Bovine Clostridium beijerinckii Enterococcus faecalis Escherichia coli k12 Frozen Glycerol Heart Human Oral cavity Prevotella intermedia Ruminococcus bromii Strains

Corresponding Organization : McGill University

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Variable analysis

independent variables

Bacterial strains used in the study

dependent variables

Not explicitly mentioned

control variables

Culture conditions: Anaerobic chamber (5% H2, 20% CO2, 75% N2) at 37°C
Culture media: Reduced Bovine Heart Infusion broth (rBHI)

controls

Positive control: Not mentioned
Negative control: Not mentioned

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