Immunoblot Analysis of Metabolic Proteins

Immunohistochemistry and immunoblot analysis were performed as previously described (48 (link)). In the current study, blots were incubated with LDHA (1:1,000), LDHB (1:1,000), hexokinase 1 (1:1,000), hexokinase 2 (1:1,000), Aldolase C (1:1,000), cone arrestin (1:1,000), pPKM2 (1:1,000), PKM2 (1:1,000), PKM1 (1:1,000), Pde6β (1:1,000), rhodopsin (1:1,000), rod arrestin (1:1,000), M-opsin (1:1,000), PDH (1:1,000) and actin (1:1,000) antibodies (Table S5) overnight at 4° C. The blots were then washed and incubated with HRP-coupled anti-mouse or anti-rabbit secondary antibodies (as appropriate) for 60 min at room temperature. After washing, blots were developed with enhanced SuperSignal West Dura Extended Duration Substrate (Thermo Fisher Scientific, Waltham, MA) and visualized using a Kodak Imager with chemiluminescence capability.