Rare Tumor CTC Isolation via ScreenCell

Peripheral blood was collected into ScreenCell blood collection tubes. 7.5 mL of each blood sample collected in a K2-EDTA was processed for 3 h using ScreenCell devices (Sarcelles, France) according to the manufacturer’s protocol. ScreenCell filters were washed with RPMI 1640 medium and Red Blood Lysis Buffer (MiltenyBiotec, Bologna, Italy). Then, enrichment filter-adherent CTCs were collected in RPMI medium and cell suspension filtered again. The ScreenCell microfiltration is an epitope-independent size-based device, able to capture all CTCs in the bloodstream. It has been used in CTC identification in rare tumors like hemangiopericytoma [22 (link)] as well as in more common tumors including bladder cancer [23 (link)].

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Morelli M.B., Amantini C., Rossi de Vermandois J.A., Gubbiotti M., Giannantoni A., Mearini E., Maggi F., Nabissi M., Marinelli O., Santoni M., Cimadamore A., Montironi R, & Santoni G. (2021). Correlation between High PD-L1 and EMT/Invasive Genes Expression and Reduced Recurrence-Free Survival in Blood-Circulating Tumor Cells from Patients with Non-Muscle-Invasive Bladder Cancer. Cancers, 13(23), 5989.

Publication 2021

Red Blood Lysis Buffer

Bladder cancer Blood Bloodstream Buffer Cell Device Edta Epitope Hemangiopericytoma Tumors

Corresponding Organization : Università di Camerino

Other organizations : University of Perugia, Ospedale San Donato, University of Siena, Ospedale Santa Maria alle Scotte, Sapienza University of Rome, University of Macerata, Marche Polytechnic University

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Variable analysis

independent variables

ScreenCell blood collection tubes used to collect peripheral blood samples
7.5 mL of each blood sample collected in a K2-EDTA
ScreenCell devices (Sarcelles, France) used to process the blood samples for 3 h

dependent variables

Enrichment of filter-adherent CTCs collected in RPMI medium

control variables

RPMI 1640 medium used to wash the ScreenCell filters
Red Blood Lysis Buffer (MiltenyBiotec, Bologna, Italy) used to wash the ScreenCell filters

positive controls

ScreenCell microfiltration device used in CTC identification in rare tumors like hemangiopericytoma
ScreenCell microfiltration device used in CTC identification in more common tumors including bladder cancer

negative controls

Not explicitly mentioned

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