Ana2014 cells were transfected with either 200 nM control siRNA or 200 nM RUVBL-1 targeting siRNAs (Kaneka Eurogentec S.A.) as described previously (36 (link)). The three siRNA sets were used (Table S2). Then, 24 h posttransfection, cells were collected and used to perform qRT-PCR, Western blotting, and immunofluorescence studies as described above.
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