Antibody Characterization for DNA Replication

Antibodies against Treslin, MTBP, and TopBP1 were described (Boos et al, 2011 (link), 2013 (link); Köhler et al, 2019 (link)). Anti-BrdU-FITC (556028; Becton Dickinson); anti-HA (mouse, 16B12; Covance); anti-GFP nanobodies (kind gift from Kirill Alexandrov); anti-GFP (mouse, JL-8, 632381; Clonetech), anti-Mcm2 (goat, sc-9839; Santa Cruz Biotechnology), anti-Mcm5 (rabbit, ab17967; Abcam), anti-Cdc45 (rat, 3G10; kind gift from Helmut Pospiech), anti-PCNA (mouse, sc-56; Santa Cruz Biotechnology), NHS (N-hydroxysuccinimide) sepharose (10343240; Thermo Fisher Scientific), and Protein G magnetic beads (10004D; Life Technologies).

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Ferreira P., Sanchez-Pulido L., Marko A., Ponting C.P, & Boos D. (2022). Refining the domain architecture model of the replication origin firing factor Treslin/TICRR. Life Science Alliance, 5(5), e202101088.

Publication 2022

Anti-BrdU-FITC anti-HA anti-Mcm2 anti-Mcm5 anti-PCNA Protein G magnetic beads

Antibodies Brdu Fitc Goat Mcm2 Mcm5 Mouse N hydroxysuccinimide Nanobodies Pcna Protein g Rabbit Sepharose

Corresponding Organization : University of Duisburg-Essen

Other organizations : Medical Research Council, University of Edinburgh, MRC Institute of Genetics and Molecular Medicine

Top 5 similar protocols

Variable analysis

independent variables

Antibodies against Treslin, MTBP, and TopBP1

dependent variables

Not explicitly mentioned

control variables

Anti-BrdU-FITC (556028; Becton Dickinson)
Anti-HA (mouse, 16B12; Covance)
Anti-GFP nanobodies (kind gift from Kirill Alexandrov)
Anti-GFP (mouse, JL-8, 632381; Clonetech)
Anti-Mcm2 (goat, sc-9839; Santa Cruz Biotechnology)
Anti-Mcm5 (rabbit, ab17967; Abcam)
Anti-Cdc45 (rat, 3G10; kind gift from Helmut Pospiech)
Anti-PCNA (mouse, sc-56; Santa Cruz Biotechnology)
NHS (N-hydroxysuccinimide) sepharose (10343240; Thermo Fisher Scientific)
Protein G magnetic beads (10004D; Life Technologies)

controls

Positive controls not explicitly mentioned
Negative controls not explicitly mentioned

Annotations

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