Mouse Model of Tet2 Deficiency

Tet2-KO mice (Tet2^f/f/Vav-Cre) [18 (link)] were maintained in pathogen-free conditions and their care followed MDACC Institutional Care and Use Committee approved animal use protocols and US Department of Health and Human Services guidelines. DS-5272 (provided by Daiichi-Sankyo, Chuo, Japan) was dissolved in 0.5% w/v methylcellulose 400 solution as 5 mg/ml for oral administration (O.P.). AZA was dissolved in PBS at 0.25 mg/ml for intraperitoneal injection (i.p.). Complete blood count (CBC) analysis was performed using a HORIBA ABX Pentra analyzer (HORIBA Instruments Incorporated, Irvine, CA). Animals were euthanized with CO₂ and cell suspensions of BM were analyzed using a Gollios flow cytometer (Beckman Coulter, Indianapolis, IN). Data were analyzed using the Kaluza flow cytometry analysis software (Beckman Coulter, Indianapolis, IN).