Imaging of T-bet Expressing Neurons

All animal experiments have been approved by the OIST Graduate University’s Animal Care and Use Committee (protocol 2016-151 and 2020-310). Tbx21^Cre (Haddad et al., 2013 (link)) and B6J.Cg-Gt(ROSA)26Sor^{tm95.1(CAG-GCaMP6f)Hze}/MwarJ, also known as Ai95D (Madisen et al., 2015 (link)), were originally obtained from the Jackson Laboratory (stock numbers 024507 and 028865, respectively). Tbx21^Cre::Ai95D mice were generated by crossing homozygous Tbx21^Cre and Ai95D mice, resulting in heterozygous animals used for imaging experiments. C57Bl6J mice were purchased from Japan CLEA (Shizuoka, Japan) and were acclimatised to the OIST facility for at least 1 week before they were used for experiments. All mice used in this study were adult males (8–11 weeks old at the time of surgery).