Wet cell-cultured samples were fixed in 4% methanol-free formaldehyde (Thermo Fisher Scientific, USA) overnight at 4°C. Applying paraffinization process, samples were paraffinized. 5 micron thick sections were cut from the paraffinized samples for staining.
The sections were stained separately with hematoxylin and eosin (Thermo Fisher Scientific, USA), picrosirius red (ScyTek Lab, USA) and Safranin O (ScienCell Research Lab, USA) for their histological analysis following manufacturer protocols and/or our previously defined protocols [18 (link), 29 ].
The sections were stained separately with elastin (ab21610, abeam, USA), tropoelastin (ab21600, abeam, USA), vimentin (ab92547, abeam, USA) and α=smooth muscle actin (SMA) markers for their immunohistochemical analysis following manufacturer protocols and/or our previously defined protocols [18 (link), 29 ].
Following the procedures stated above, explanted samples were fixed, paraffinized, sectioned and stained separately with DAPI (Thermo Fisher Scientific, USA) for their immunofluorescent analysis [18 (link), 25 (link), 30 ].
The sections were stained separately with hematoxylin and eosin (Thermo Fisher Scientific, USA), picrosirius red (ScyTek Lab, USA) and Safranin O (ScienCell Research Lab, USA) for their histological analysis following manufacturer protocols and/or our previously defined protocols [18 (link), 29 ].
The sections were stained separately with elastin (ab21610, abeam, USA), tropoelastin (ab21600, abeam, USA), vimentin (ab92547, abeam, USA) and α=smooth muscle actin (SMA) markers for their immunohistochemical analysis following manufacturer protocols and/or our previously defined protocols [18 (link), 29 ].
Following the procedures stated above, explanted samples were fixed, paraffinized, sectioned and stained separately with DAPI (Thermo Fisher Scientific, USA) for their immunofluorescent analysis [18 (link), 25 (link), 30 ].
