Isolating and Treating pmATII Cells

The pmATII cells were isolated from mice as previously described [8 (link)]. The pmATII cells were seeded in 12 well-tissue culture plates and cultured in DMEM-F12 supplemented with 10% FCS, 2 mM 1-glutamine, 1% penicillin/streptomycin, 3.6 mg/ml glucose and 10 mM HEPES for 24 h to allow attachment. Cells were then cultured for 12 h in fresh 0.1% FCS containing medium. Subsequently, cells were pre-treated with Nintedanib (1 μM) (Selleck, Houston, TX) or Pirfenidone (500 μM) (Selleck, Houston, TX) or respective DMSO control for 48 h.

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Lehmann M., Buhl L., Alsafadi H.N., Klee S., Hermann S., Mutze K., Ota C., Lindner M., Behr J., Hilgendorff A., Wagner D.E, & Königshoff M. (2018). Differential effects of Nintedanib and Pirfenidone on lung alveolar epithelial cell function in ex vivo murine and human lung tissue cultures of pulmonary fibrosis. Respiratory Research, 19, 175.

Publication 2018

Nintedanib Pirfenidone

Cells Dmso Glucose Glutamine Hepes Mice Nintedanib Penicillin Pirfenidone Streptomycin Tissue

Corresponding Organization :

Other organizations : German Center for Lung Research, Helmholtz Zentrum München, Ludwig-Maximilians-Universität München, Asklepios Fachkliniken München-Gauting

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Variable analysis

independent variables

Nintedanib (1 μM)
Pirfenidone (500 μM)

dependent variables

Not explicitly mentioned

control variables

DMSO control

Annotations

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