Measuring cAMP and Ca2+ in Opsin-HEK Cells
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Corresponding Organization :
Other organizations : Osaka City University
Variable analysis
- Light stimuli: Broadband green LED light, narrow-band violet (410 nm) and blue (430 nm) LED lights
- Intracellular cAMP levels in opsin-expressing HEK293S cells (measured using the GloSensor cAMP assay)
- Intracellular Ca2+ levels in opsin-expressing HEK293S cells (measured using the aequorin assay)
- Opsin-expressing HEK293S cells (used as the experimental system)
- GloSensor cAMP assay (used to measure intracellular cAMP levels)
- Aequorin assay (used to measure intracellular Ca2+ levels)
- Not explicitly mentioned
- Not explicitly mentioned
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