Kinetics of CAIX-Targeting Radiotracer Binding

The kinetics of binding of ^99mTc-(HE)₃-ZCAIX:1 to living CAIX-expressing SK-RC-52 renal carcinoma cells was measured at 4°C using LigandTracer Yellow (Ridgeview Instruments, Vänge, Sweden) according to the established method (42 (link)). The LigandTracer device records in real-time kinetics of binding to and dissociation of radiolabeled tracers from living cells. TraceDrawer software (Ridgeview Instruments) permits to calculate both the association and dissociation rates and, based on that, the affinity of radiolabeled conjugates is determined. In order to cover the concentration span needed for proper affinity estimation, three increasing concentrations of ^99mTc-(HE)₃-ZCAIX:1 (30, 90 and 150 nM) were used in each affinity assay.

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HONARVAR H., GAROUSI J., GUNNERIUSSON E., HÖIDÉN-GUTHENBERG I., ALTAI M., WIDSTRÖM C., TOLMACHEV V, & FREJD F.Y. (2014). Imaging of CAIX-expressing xenografts in vivo using 99mTc-HEHEHE-ZCAIX:1 Affibody molecule. International Journal of Oncology, 46(2), 513-520.

Publication 2014

LigandTracer Yellow TraceDrawer software

Assay Caix Cells Device Kinetics Renal cells carcinoma 1

Corresponding Organization :

Other organizations : Uppsala University, Affibody (Sweden), Uppsala University Hospital

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Variable analysis

independent variables

Concentration of 99mTc-(HE)3-ZCAIX:1 (30 nM, 90 nM, 150 nM)

dependent variables

Kinetics of binding of 99mTc-(HE)3-ZCAIX:1 to living CAIX-expressing SK-RC-52 renal carcinoma cells
Association and dissociation rates of 99mTc-(HE)3-ZCAIX:1
Affinity of 99mTc-(HE)3-ZCAIX:1

control variables

Temperature (4°C)
LigandTracer Yellow device
TraceDrawer software

controls

No positive or negative controls were explicitly mentioned in the input protocol.

Annotations

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