Measurement of NF-κB Activity in Caco-2 Cells
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Corresponding Organization : VA Eastern Colorado Health Care System
Other organizations : University Hospital Bonn, Montana State University
Variable analysis
- Transfection of NF-κB luciferase reporter plasmid (500 ng) and Renilla reporter plasmid (1 ng) into Caco-2 cells
- Luciferase activity determined at 16 h using Promega dual luciferase reagents
- Caco-2 cell line
- Lipofectamine LTX Reagent (Thermo Scientific) used for transfection
- Experiments performed in triplicate
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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