Identification and Characterization of tEPEC Strains
Strains were cultured from the feces of infants or children who had acute MSD or who were recruited as healthy controls; tEPEC strains had been identified as described [16 (link)]. Individual colonies were shipped from countries of origin to Baltimore, Maryland, USA and verified by PCR, using primers listed in S1 Table as previously described [9 (link)] for bfpA and escV to identify tEPEC, and stxA1 and stxA2 to identify and exclude Shiga-toxin producing E. coli. PCR using the same conditions and primers also listed in S1 Table was used to detect nleG clusters in a larger collection of 63 NSI and 56 AI unmatched tEPEC and atypical EPEC (aEPEC, bfpA-/escV+/stx-) GEMS isolates.
PCR using primers for bfpA, escV, stxA1, and stxA2 to identify tEPEC and exclude Shiga-toxin producing E. coli
PCR using the same conditions and primers listed in S1 Table to detect nleG clusters in a collection of 63 NSI and 56 AI unmatched tEPEC and atypical EPEC (aEPEC, bfpA-/escV+/stx-) GEMS isolates
dependent variables
Identification of tEPEC strains
Identification of Shiga-toxin producing E. coli
Detection of nleG clusters in tEPEC and aEPEC GEMS isolates
control variables
Strains cultured from the feces of infants or children who had acute MSD or who were recruited as healthy controls
Primers listed in S1 Table
positive controls
Not explicitly mentioned
negative controls
Not explicitly mentioned
Annotations
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