TRPM7 knockdown experiments were performed as described previously [22 (link);23 (link)]. Briefly, special siRNA targeting nucleotides 406–426 of human TRPM7 (NM_017672) was synthesized from Invitrogen. Cells were treated with 30 nM siRNA using transfection reagent lipofectamineTM RNAiMAX (Invitrogen) according to the manufacturer’s instructions. Non-targeting siRNA (Invitrogen) was used as a negative control
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TRPM7 Knockdown Experiments Protocol
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Corresponding Organization :
Other organizations : Morehouse School of Medicine, Neurological Surgery, Oregon Health & Science University, Zhejiang University, Legacy Health, Sun Yat-sen University, Anhui Medical University
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Variable analysis
independent variables
- TRPM7 knockdown using siRNA
- Unspecified
- Cell type/line
- Transfection reagent (Lipofectamine RNAiMAX)
- Concentration of siRNA (30 nM)
- Positive control: Non-targeting siRNA
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