All oligonucleotide sequences and modification patterns synthesized in-house are summarized in Supplementary Table S1. Accell siRNA (Thermo Fisher) targeting HTT was purchased and used without further purification. Clonidine (Sigma–Aldrich), Retro-1 (Sigma–Aldrich) and Salubrinal (Sigma–Aldrich) were dissolved in DMSO and stored as 10 mM stock solutions at −20°C. Unless otherwise specified, all oligonucleotides and small molecules were diluted in OptiMEM (Gibco) prior to administration in vitro.
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Oligonucleotide and Small Molecule Protocols
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Other organizations : University of Massachusetts Chan Medical School
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Variable analysis
independent variables
- Clonidine (Sigma–Aldrich)
- Retro-1 (Sigma–Aldrich)
- Salubrinal (Sigma–Aldrich)
- Not explicitly mentioned
- All oligonucleotide sequences and modification patterns synthesized in-house are summarized in Supplementary Table S1
- Accell siRNA (Thermo Fisher) targeting
HTT was purchased and used without further purification - All oligonucleotides and small molecules were diluted in OptiMEM (Gibco) prior to administration
in vitro
- Accell siRNA (Thermo Fisher) targeting
HTT was used as a positive control
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