Dichromate within the ethanoic acid is reduced to chromic acetate when heated within the presence of peroxide (H2O2), with the formation of per chromic acid as an unstable intermediate. The chromic acetate, thus produced was measured spectrophotometrically at 570 nm. The activity of catalase within the tissue samples is expressed as µ moles of H2O2 consumed/min/mg protein (23) .
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Spectrophotometric Quantification of Catalase
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Corresponding Organization : University of Madras
Other organizations : Czech Academy of Sciences, Institute of Physiology
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Variable analysis
independent variables
- Heating of dichromate within ethanoic acid in the presence of peroxide (H2O2)
- Formation of chromic acetate
- Spectrophotometric measurement of chromic acetate at 570 nm
- Activity of catalase within the tissue samples expressed as µ moles of H2O2 consumed/min/mg protein
- Not explicitly mentioned
- No positive or negative controls specified
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