Surgical Liver Exposure and Manipulation

Mice were placed on a heating pad in the supine position. A 1 cm skin incision was made in the left subcostal area, followed by a 1 cm incision in the peritoneum to expose the liver. Using a cotton swab, the left lobe of the liver was moved outside the body and placed on a nonwoven absorbent fabric sheet for the injection or implantation. After the injection or surgical implantation, the liver was returned within the body, and the abdominal incision was closed in 2 layers with 5-0 polydioxanone absorbable thread (AD Surgical, Sunnyvale, CA, USA).

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Kageyama K., Ohara M., Saito K., Ozaki S., Terai M., Mastrangelo M.J., Fortina P., Aplin A.E, & Sato T. (2017). Establishment of an orthotopic patient-derived xenograft mouse model using uveal melanoma hepatic metastasis. Journal of Translational Medicine, 15, 145.

Publication 2017

Abdominal Body Cotton Implantation Liver Mice Peritoneum Polydioxanone Skin Surgical

Corresponding Organization :

Other organizations : Thomas Jefferson University, Sidney Kimmel Cancer Center

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Protocol cited in 3 other protocols

Variable analysis

independent variables

Injection or surgical implantation procedure performed on the left lobe of the liver

dependent variables

Outcome of the injection or surgical implantation procedure

control variables

Mice placed on a heating pad in the supine position
1 cm skin incision made in the left subcostal area
1 cm incision made in the peritoneum to expose the liver
Left lobe of the liver moved outside the body and placed on a nonwoven absorbent fabric sheet
Abdominal incision closed in 2 layers with 5-0 polydioxanone absorbable thread

controls

No positive or negative controls were explicitly mentioned in the input protocol.

Annotations

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