HUVECs were obtained from the BeNa Culture Collection (Beijing, China). The cells were cultured in Dulbecco’s modified Eagle’s Medium (DMEM) supplemented with 10% fetal bovine serum (Gibco, United States), streptomycin (100 μg/ml), and penicillin (100 IU/ml) in a humidified atmosphere containing 5% CO2/95% air at 37°C. The culture medium was replaced every 2 days.
In the experiment, HUVECs were pretreated with rosuvastatin (MedChemExpress, United States; 2.5, 5, and 10 μM) for 2 h. The concentrations were determined prior to the experiment with a drug concentration gradient (Wang et al., 2010 (link)). rosuvastatin was dissolved at a certain concentration in dimethyl sulfoxide (DMSO) (Piconi et al., 2008 (link)); the final concentration of DMSO was always lower than 0.01%, which had been shown to have no effect on cell viability (Gao et al., 2008 (link)). The cells were then exposed to H2O2 (750 μM) for 24 h (Chen et al., 2014 (link)). The final concentrations of H2O2 were determined by the experiment with a H2O2 concentration gradient.
In the experiment, HUVECs were pretreated with rosuvastatin (MedChemExpress, United States; 2.5, 5, and 10 μM) for 2 h. The concentrations were determined prior to the experiment with a drug concentration gradient (Wang et al., 2010 (link)). rosuvastatin was dissolved at a certain concentration in dimethyl sulfoxide (DMSO) (Piconi et al., 2008 (link)); the final concentration of DMSO was always lower than 0.01%, which had been shown to have no effect on cell viability (Gao et al., 2008 (link)). The cells were then exposed to H2O2 (750 μM) for 24 h (Chen et al., 2014 (link)). The final concentrations of H2O2 were determined by the experiment with a H2O2 concentration gradient.
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