Mitochondrial Superoxide Detection Using MitoSox Red

Mitochondrial superoxide was determined as previously described by using Mitosox Red (Life technologies)[22 (link)–24 (link)]. Neurons were incubated with 2 μM Mitosox Red and 200 nM mitotracker green (Life Technology) for 30 minutes followed by washing. The images of Mitosox Red staining were collected on a Nikon inverted confocal microscope with on stage incubator. The intensity was subsequently analyzed by using Nikon NIS Advanced Research software.

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Lu L., Guo L., Gauba E., Tian J., Wang L., Tandon N., Shankar M., Beck S.J., Du Y, & Du H. (2015). Transient Cerebral Ischemia Promotes Brain Mitochondrial Dysfunction and Exacerbates Cognitive Impairments in Young 5xFAD Mice. PLoS ONE, 10(12), e0144068.

Publication 2015

Mitosox Red mitotracker green inverted confocal microscope NIS Advanced Research software

Confocal microscope Mitochondrial Neurons Superoxide

Corresponding Organization : The University of Texas at Dallas

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Variable analysis

independent variables

Mitochondrial superoxide

dependent variables

Mitochondrial superoxide intensity

control variables

Neurons incubated with 2 μM MitoSox Red and 200 nM mitotracker green for 30 minutes
Images of Mitosox Red staining collected on a Nikon inverted confocal microscope with on stage incubator
Intensity analyzed using Nikon NIS Advanced Research software

controls

No positive or negative controls were explicitly mentioned in the provided information.

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