Glucose levels were measured by spotting blood on glucose strips and reading them by an enzyme electrode method using the Ascensia ELITE XL blood glucose meter (Bayer Consumer Care AG, Basel, Switzerland). Plasma insulin concentrations were measured using a Rat/Mouse Insulin ELISA kit according to the manufacturer’s instructions (Millipore Corporation, Billerica, MA, USA). These parameters have been described in the same rat cohort in previous works (23 (link), 24 (link)).
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