Culturing Human Leukemia and Liver Cancer Cells

The human chronic myeloid leukemia (CML) cell line K562, as well as a CRISPRi derivative of this cell line constitutively expressing a catalytically inactive Cas9 fused to a KRAB effector domain (dCas9-KRAB)[17 (link)] was kindly provided by the Innovative Genomics Institute, UCSF. K562 cell lines and human hepatocellular carcinoma Huh7 cells (ATCC) were cultured in RPMI 1640 medium (Life Technologies, CARLSBAD, CA, USA) supplemented with 0.2 mM L-glutamine (Glutamax, Life Technologies), 10% FBS (F4135, Sigma-Aldrich, St. Louis, MO, USA), and antibiotics (Penicillin/Streptomycin, 0.1 mg/mL, Gibco) unless otherwise stated. HEK293T cells (UC Berkeley Cell culture facility) were maintained in DMEM (Gibco) supplemented with 10% FBS (Seradigm) and antibiotics unless otherwise stated. Cells tested negative for mycoplasma infection before use.

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Liaud N., Horlbeck M.A., Gilbert L.A., Gjoni K., Weissman J.S, & Cate J.H. (2019). Cellular response to small molecules that selectively stall protein synthesis by the ribosome. PLoS Genetics, 15(3), e1008057.

Publication 2019

RPMI 1640 medium L-glutamine (Glutamax, F4135 Penicillin/Streptomycin DMEM

Antibiotics Cell culture Cell lines Cells Chronic myeloid leukemia Cultured medium Glutamine Hepatocellular carcinoma Human Mycoplasma infection Penicillin Streptomycin

Corresponding Organization : Lawrence Berkeley National Laboratory

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Variable analysis

independent variables

Cell line (K562 and CRISPRi derivative of K562 cell line)
Cell culture media (RPMI 1640 and DMEM)

dependent variables

Cellular response (not explicitly mentioned)

control variables

L-glutamine (0.2 mM)
FBS (10%)
Antibiotics (Penicillin/Streptomycin, 0.1 mg/mL)
Mycoplasma infection (tested negative before use)

controls

Positive control: Not specified
Negative control: Not specified

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