To block the histone deacetylase activity, tadpoles were incubated with TSA (Sigma-Aldrich) (Tseng et al., 2011 (link)), MS-275 or MGCD0103 (Selleck) (Bolden et al., 2006 (link); Bradner et al., 2010 (link)) in Steinberg's solution for 24–48 h.
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