Quantification of FGF21 and Insulin in Plasma

Concentrations of mouse and recombinant human FGF21 in plasma were determined by specific ELISAs according to the procedure recommended by the manufacturer (no. RD291108200R, mouse and rat FGF-21 ELISA; no. RD191108200R, human FGF21 ELISA; BioVendor, Brno, Czech Republic) and as described previously [3 (link), 4 (link)]. Plasma insulin concentrations were determined with an ELISA (80-INSMSU-E01; ALPCO Diagnostics, Salem, NH, USA) [37 (link)].

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Laeger T., Baumeier C., Wilhelmi I., Würfel J., Kamitz A, & Schürmann A. (2017). FGF21 improves glucose homeostasis in an obese diabetes-prone mouse model independent of body fat changes. Diabetologia, 60(11), 2274-2284.

Publication 2017

RD291108200R mouse and rat FGF-21 ELISA human FGF21 ELISA 80-INSMSU-E01

Diagnostics Elisa Fgf21 Human Insulin Mouse Plasma

Corresponding Organization :

Other organizations : German Institute of Human Nutrition, German Center for Diabetes Research, Heinrich Heine University Düsseldorf, Deutsches Diabetes-Zentrum e.V.

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Variable analysis

independent variables

Concentrations of mouse and recombinant human FGF21 in plasma

dependent variables

Plasma insulin concentrations

control variables

Not explicitly mentioned

controls

No positive or negative controls were specified by the authors.

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