Immunofluorescence Staining with Diverse Antibodies

The following primary antibodies were used: chicken anti-HTP-3 (1:1000, (46 (link))), chicken anti-GFP (1:250, (Abcam)), mouse anti-GFP (1:500, (Roche)), rabbit anti-GFP (1:500, (47 (link))), mouse anti-HA (1:1000, (Clone 16B12; Covance)), rabbit anti-MSH-5 (1:10 000, (SDIX)), rabbit anti-RAD-51 (1:500, (48 (link))), rat anti-RAD-51 (1:200, (49 (link))), guinea pig anti-SUN-1 pS24 (1:700, (50 (link))), guinea pig anti-SUN-1 pS8(1:1000, (50 (link))), guinea pig anti-SYP-1 (1:200, (51 (link))). Secondary antibodies used were Alexa Fluor 405 (1:100), 488 (1:400), 555 (1:400), and 647 (1:200)-conjugated goat antibodies raised against the appropriate species (Life Technologies).

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Yamaya K., Wang B., Memar N., Odiba A.S., Woglar A., Gartner A, & Villeneuve A.M. (2023). Disparate roles for C. elegans DNA translocase paralogs RAD-54.L and RAD-54.B in meiotic prophase germ cells. Nucleic Acids Research, 51(17), 9183-9202.

Publication 2023

chicken anti-GFP mouse anti-GFP rabbit anti-GFP mouse anti-HA Alexa Fluor 405

Antibodies Chicken Clone Goat Guinea pig Mouse Rabbit

Corresponding Organization :

Other organizations : Stanford University, Guangxi Academy of Sciences, Ulsan National Institute of Science and Technology, École Polytechnique Fédérale de Lausanne

Top 5 similar protocols

Variable analysis

independent variables

Chicken anti-HTP-3 (1:1000, (46 (link)))
Chicken anti-GFP (1:250, (Abcam))
Mouse anti-GFP (1:500, (Roche))
Rabbit anti-GFP (1:500, (47 (link)))
Mouse anti-HA (1:1000, (Clone 16B12; Covance))
Rabbit anti-MSH-5 (1:10 000, (SDIX))
Rabbit anti-RAD-51 (1:500, (48 (link)))
Rat anti-RAD-51 (1:200, (49 (link)))
Guinea pig anti-SUN-1 pS24 (1:700, (50 (link)))
Guinea pig anti-SUN-1 pS8(1:1000, (50 (link)))
Guinea pig anti-SYP-1 (1:200, (51 (link)))

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

Positive controls: Not specified
Negative controls: Not specified

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