Lamina propria Mononuclear Cells (LPMC) were prepared from murine intestines by enzymatic digestion as previously described (66 (link)). Briefly, cells were isolated from colons, after removal of epithelial cells, by enzymatic digestion with 200mg/ml fungizone, 1.25 mg/ml collagenase D (Roche Diagnostics), 0.85 mg/ml collagenase V (Sigma-Aldrich), 1 mg/ml dispase (Life Technologies), and 30 U/ml DNaseI (Roche Diagnostics) in complete RPMI 1640 for 30–40 min in a shaking incubator until complete digestion of the tissue. After isolation, cells were passed through a 40μm cell strainer before use (BD biosciences).
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