Immunohistochemistry was performed in tumor tissues using the streptavidin-peroxidase (SP) method (Ultra Sensitive TM-SP) as described previously [32 (link)]. Scores were independently performed by two pathologists blinded to the identity of the tissue specimens as described [33 (link)]. The RUNX2 positive cells were photographed with Inverted Research Microscope (Olympus).
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