Quantification of miRNAs using TaqMan assays

Total RNA was reverse transcribed using TaqMan advanced miRNA cDNA synthesis kit (Life Technologies, Waltham, MA, USA). Quantification of miRNAs was completed using the TaqMan fast advanced PCR master mix in conjunction with TaqMan miRNA expression assays (Life Technologies, Waltham, MA, USA). For miRNA normalization, miR-191-5p was used as an endogenous control [23 (link), 24 (link)]. The ΔΔC_t method was used for analysis of real-time data to obtain a miRNA fold-change relative to miR-191-5p expression. Mann-Whitney-U was used for statistical analysis.

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Awamleh Z., Gloor G.B, & Han V.K. (2019). Placental microRNAs in pregnancies with early onset intrauterine growth restriction and preeclampsia: potential impact on gene expression and pathophysiology. BMC Medical Genomics, 12, 91.

Publication 2019

TaqMan advanced miRNA cDNA synthesis kit TaqMan fast advanced PCR master mix

Assays Cdna Mirna Synthesis

Corresponding Organization :

Other organizations : Children’s Health Research Institute, Western University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

MiRNA expression levels

control variables

MiR-191-5p expression used as an endogenous control for miRNA normalization

controls

Positive control: None mentioned
Negative control: None mentioned

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