Optogenetic Manipulation of Rodent Behavior

Optogenetic manipulation was performed using nVoke 2.0 (Inscopix). For optogenetic activation, 20 Hz, 60-pulse trains (5 ms each) of LED light (620 ± 30 nm, 20 mW/mm²) were initiated every 30 s during the light on epoch^{48 (link)}. For optogenetic inhibition, constant LED light (620 ± 30 nm, 5 mW/mm²) was delivered during the light on epoch^{49 (link)}. The EthoVision XT 15 software and a mini-IO box system (Noldus) were used to record live tracking of mice, and to trigger the laser on during specific behaviors.

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Kin K., Francis-Oliveira J., Kano S.I, & Niwa M. (2023). Adolescent stress impairs postpartum social behavior via anterior insula-prelimbic pathway in mice. Nature Communications, 14, 2975.

Publication 2023

nVoke 2.0 EthoVision XT 15 mini-IO box system

Inhibition Light Mice Optogenetic Pulse Trigger

Corresponding Organization :

Other organizations : University of Alabama at Birmingham

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Variable analysis

independent variables

Optogenetic activation: 20 Hz, 60-pulse trains (5 ms each) of LED light (620 ± 30 nm, 20 mW/mm^2)
Optogenetic inhibition: Constant LED light (620 ± 30 nm, 5 mW/mm^2)

dependent variables

Live tracking of mice behaviors

control variables

The EthoVision XT 15 software and a mini-IO box system (Noldus) were used to trigger the laser on during specific behaviors

controls

Positive control: Optogenetic activation
Negative control: Optogenetic inhibition

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