Example 2

Round 3-D inserts (having three micro-channels of 3 mm width by 20 mm length or 25 mm length by 10 mm height) or square 3-D inserts (having three micro-channels of 15 mm length) were seeded with 50,000 or 100,000 P6 human bone marrow-derived mesenchymal stem cells per channel in 200 μl of culture media in a tissue culture dish on a rocking platform. Rocking frequency was 5 rpm and 15° tilt. Cells were cultured for two days. FIGS. 4A-4D show cell aggregates at 24 and 48 hours.

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