Histone Modification Analysis Protocol

The following antibodies were used in this study: anti-KDM2A (12 (link)), anti-KDM2B (15 (link)), anti-BRG1 (EPNCIR111A, Abcam), anti-H3, anti-H3K36me1, anti-H3K36me2 (12 (link)), anti-H3K36me3, anti-H4 (L64C1, Cell Signalling), anti-H2AK119ub1 (D27C4, Cell Signalling), anti-H3K27me3 (45 (link)), anti-Rbp1-NTD (D8L4Y, Cell Signalling), anti-Rbp1-CTD-Ser5P (D9N5I, Cell Signalling), anti-Rbp1-CTD-Ser2P (E1Z3G, Cell Signalling). Anti-H3 and anti-H3K36me antibodies were prepared in-house by rabbit immunization with synthetic peptides (PTU/BS Scottish National Blood Transfusion Service) and antibodies were purified on peptide affinity columns.

Free full text: Click here

Turberfield A.H., Kondo T., Nakayama M., Koseki Y., King H.W., Koseki H, & Klose R.J. (2019). KDM2 proteins constrain transcription from CpG island gene promoters independently of their histone demethylase activity. Nucleic Acids Research, 47(17), 9005-9023.

Publication 2019

anti-BRG1 (EPNCIR111A, anti-H3K36me2 anti-H3K36me3 anti-H3K27me3

Anti antibodies Antibodies Blood transfusion Brg1 House rabbit Immunization Kdm2b Peptide

Corresponding Organization :

Other organizations : University of Oxford, RIKEN Center for Integrative Medical Sciences, Kazusa DNA Research Institute, Japan Science and Technology Agency

Top 5 similar protocols

Variable analysis

independent variables

Anti-KDM2A
Anti-KDM2B
Anti-BRG1
Anti-H3
Anti-H3K36me1
Anti-H3K36me2
Anti-H3K36me3
Anti-H4
Anti-H2AK119ub1
Anti-H3K27me3
Anti-Rbp1-NTD
Anti-Rbp1-CTD-Ser5P
Anti-Rbp1-CTD-Ser2P

dependent variables

Unspecified

control variables

Unspecified

positive controls

Unspecified

negative controls

Unspecified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!