Visualizing NF-κB Translocation in NKILA Cells

1 × 10³ S26 cells overexpressing NKILA or NKILA shRNA were cultured on coverslips overnight prior to the experiment. After fixing with 4% paraformaldehyde, IF was done and imaged as previously reported [50 (link)], primary antibodies against P65 was used, followed by FITC-conjugated secondary antibodies (Invitrogen, Carlsbad, CA). Sc-3060(Santa Cruz, CA) and JSH-23(Millipore, Billerica, MA) are drugs which inhibit NF-kB nuclear translocation. 30min before specified treatment,10uM Sc-3060 and 5uM JSH-23 were added into the culture.

Free full text: Click here

Zhang W., Guo Q., Liu G., Zheng F., Chen J., Huang D., Ding L., Yang X., Song E., Xiang Y, & Yao H. (2019). NKILA represses nasopharyngeal carcinoma carcinogenesis and metastasis by NF-κB pathway inhibition. PLoS Genetics, 15(8), e1008325.

Publication 2019

FITC-conjugated secondary antibodies Sc-3060 JSH-23

Antibodies Cells Drugs Fitc Inhibit Jsh 23 Nf kb Paraformaldehyde Sc 3060 Shrna Translocation

Corresponding Organization : Sun Yat-sen University Cancer Center

Top 5 similar protocols

Variable analysis

independent variables

Overexpression of NKILA
Knockdown of NKILA using shRNA

dependent variables

Localization of P65 (NF-kB subunit)

control variables

Number of S26 cells (1 × 10^3)
Duration of cell culture (overnight)
Fixation with 4% paraformaldehyde
Primary antibody against P65
FITC-conjugated secondary antibody

controls

Positive control: Sc-3060 (NF-kB nuclear translocation inhibitor)
Positive control: JSH-23 (NF-kB nuclear translocation inhibitor)

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!