Cell viability was measured using an MTT assay kit (Amresco, USA) as per the manufacturer’s instructions. Briefly, the MTT solution was added to the cultured cells, and following incubation at 37 °C for 4 hours, the supernatants were removed and the formazan crystals were dissolved in 150 μl DMSO. The color was allowed to develop for ten minutes, and the absorbance was measured at 490 nm. The experiment was repeated three times, and the method has been described in our previous study34 (link).
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